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Antibodies bound to the blocking peptide now not bind on the epitope around the concentrate on protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, one can see which staining is distinct.
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This information outlines more doable optimization of cellular and immunoassay parameters to make sure the absolute best results are...
This information outlines further more attainable optimization of mobile and immunoassay parameters to make sure the best possible results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind exclusively for the target antibody and block antibody binding. These peptide ordinarily has the epitope acknowledged by the antibody.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which lead to the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions being a regulatory subunit of CDK2, whose activity is required here for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells progress through S period.
Overexpression of the gene has long been observed in several tumors, which ends up in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was uncovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important part in selling cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two more splice variants were being reported but comprehensive nucleotide sequence data is not really yet readily available.
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